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We have measured the electrophoretic mobility of carboxylated polystyrene (PS) microspheres, in different electrolytes, to determine the electrokinetic coupling factors, zeta potentials, and the electrical double layer thicknesses by streaming potential measurements. The microsphere solution was prepared by mixing 0.1 mM PS with PS poly(ethylene glycol) (PEG) aqueous solution. Addition of NaCl and alkyltrimethyl ammonium bromide (TMAB) was found to be very effective in lowering the microsphere zeta potential from ca. 40 to ca. -30 mV. The zeta potential of 0.1 mM PS was -25 mV in the presence of 0.01 mM TMAB. When the salt concentration was increased to 0.5 mM, the zeta potential of 0.1 mM PS was below -30 mV. The electric double layer thickness was determined to be 1.3 nm by fitting the PS zeta potential as a function of the distance between the microsphere and a charged planar surface. It was found that PS can be used as a model system for predicting the electrokinetic coupling factors between polymeric microspheres and charged insulators, such as porous media. The coulombic coupling factors were determined from streaming current measurements in 0.1 M NaCl solution for various PS microsphere sizes, and were found to be between 0.7 and 1.0 for sphere diameters less than 2 microm.

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